中日未来会有战争吗:请翻译生物专业英语，谢谢

The PCR amplification reaction conditions were as follows: 1 cycle of 94 .C for 5 min; 35 cycles of 94 .C for 30 s,
60 .C for 30 s, and 72 .C for 30 s; and 1 cycle of 72 .C for 5 min. The amplified PCR products were cloned into pGEM-T vector (Promega), and sequencing was
performed at the Immunomodulation Research Center, Korea, on an automatic DNA sequencer (Applied Biosystems, Inc., USA) according to the dye terminator
procedure with forward and reverse primers and overlapping primers designed from the sequencing results. Every nucleotide position in the MCP gene was
determined at least twice from each DNA strand.
The DNA and the deduced amino acid sequences were compared with the GenBank/EMBL databases using BLAST. Sequences were aligned using
CLUSTAL W [23] and then the phylogenetic tree was constructed with the TreeView [20]. The phylogenetic relationships among species were determined
using the neighbor-joining method [22] and the reliability of the NJ tree was inferred using the Felsentein [6] bootstrap method with 1000 replicates.