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Specific activity of the α-D-galactosidase still increased, showing 24% increase in activity. Grape flesh subjected by gibberellic acid (100ppm) resulted in the increased activity against a nat ural substrate , stachyose, showing 55% increase in activity from the grapes treated with 100ppm of gibberellic acid. Other natural substrates ,such as melibiose and raffinose, were also considerably hydrolyzed, and the extent was similar to that of stachyose hydrolysis. During postharvest storage , α-D-galactosidase activity in the grape flesh increased by 51% after 20 adys and then declined slowly.
Gibberellins are a class of endogenous phytohormones that regulate a wide range of developmental processes in plants, including germination ,stem elongation, and flower development .Most molecular studies on the mechanism of auxin and gibberellic acid action have been focused on the induction of cell elongation in vegetative tissues such as stems , coleoptiles, hypocotyls, and epicotyls. Some of these phytohormone induced genes have been characterized ,and the functions of proteins have been investigated. In phytohormone-induced gene expression ,the most widely investigated protein includes the induction of α-amylase by gibberellins in gibberellins in cereal germinants. Other researches have shown that gibberellins also influence the biosynthesis of various hydrolytic enzymes involved in protein and lipid degradations. Gibberellic acid has been routinely used in seedless bunch grape production and can be used to increase berry and bunch sizes .Treatment to some fruit with gibberellic acid also delay ripening. Β- Galactosidase that may facilitate the cell growth was shown to be increased by auxin in Avena and etiolated pea stems. Galactose elicits various physiological responses related with ethylene and auxin from mung bean hypocotyls. Avena coleoptiles. And tobacco leaf discs. On the analogy or these findings, treatment of gibberellin as one of phytohomones could cause some variations of glycosidases in the grape berry in relation with the plant growth regulation. Despite wide-spread investigation on the role or mechanism of gibberellic acid in plants, induction of glycosidase by the plant growth regulator has been relatively less focused on fruits. Recently ,the expression of invertase by gibberellic acid was reported in relation with the berry development , and cell compartmentation. In order to understand the role of gibberellic acid fruits ripening and mechanism of proteins expressed, it is necessary to assess glycosidases induced by the phytohormone .Thus, we report here some characteristics of glycosidase ,that was expressed in the grape flesh by the application of gibberellic acid.